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pbmcs derived from buffy coats  (Research Blood Components LLC)

 
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    Research Blood Components LLC pbmcs derived from buffy coats
    Pbmcs Derived From Buffy Coats, supplied by Research Blood Components LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbmcs+derived+from+buffy+coats/human+pbmcs+isolated+via+ficoll+gradient+density+separation+from+healthy+donor+buffy+coats/pmc05654656-60-8-12
    Average 90 stars, based on 1 article reviews
    pbmcs derived from buffy coats - by Bioz Stars, 2026-10
    90/100 stars

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    Derivative Assay:

    Article Title: CD151 expression is associated with a hyperproliferative T cell phenotype
    Article Snippet: .. For some of the studies, PBMCs derived from buffy coats were used (Research Blood Components, LLC; Brighton, MA, U.S.A.). .. PBMCs were isolated from peripheral blood using Ficoll−Paque Plus density gradient centrifugation (GE Healthcare Life Sciences, Uppsala, SE) and were cryopreserved.



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    peripheral blood mononuclear cells (pbmcs) derived from a buffy coat  (Sanquin)
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    primers used.
    Peripheral Blood Mononuclear Cells (Pbmcs) Derived From A Buffy Coat, supplied by Sanquin, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pbmcs derived from buffy coats  (Research Blood Components LLC)
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    Research Blood Components LLC pbmcs derived from buffy coats
    primers used.
    Pbmcs Derived From Buffy Coats, supplied by Research Blood Components LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbmcs+derived+from+buffy+coats/human+pbmcs+isolated+via+ficoll+gradient+density+separation+from+healthy+donor+buffy+coats/pmc05654656-60-8-12
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    primers used.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Diabetes Medication Metformin Inhibits Osteoclast Formation and Activity in In Vitro Models for Periodontitis

    doi: 10.3389/fcell.2021.777450

    Figure Lengend Snippet: primers used.

    Article Snippet: For osteoclastogenesis assays, peripheral blood mononuclear cells (PBMCs) derived from a buffy coat (Sanquin, Amsterdam) were added as source containing osteoclast precursors.

    Techniques: Sequencing, Amplification

    Outline of experiments. Timeline in days. For osteogenesis and osteoclastogenesis co culture experiments, periodontal ligament cells (PLC) were cultured 1 day before the start of the experiment. Metformin was added throughout all experiments. Analyses in italics at the days of sampling. PBMCs: peripheral blood mononuclear cells.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Diabetes Medication Metformin Inhibits Osteoclast Formation and Activity in In Vitro Models for Periodontitis

    doi: 10.3389/fcell.2021.777450

    Figure Lengend Snippet: Outline of experiments. Timeline in days. For osteogenesis and osteoclastogenesis co culture experiments, periodontal ligament cells (PLC) were cultured 1 day before the start of the experiment. Metformin was added throughout all experiments. Analyses in italics at the days of sampling. PBMCs: peripheral blood mononuclear cells.

    Article Snippet: For osteoclastogenesis assays, peripheral blood mononuclear cells (PBMCs) derived from a buffy coat (Sanquin, Amsterdam) were added as source containing osteoclast precursors.

    Techniques: Co-Culture Assay, Cell Culture, Sampling

    Graphical abstract. Diabetes and periodontitis are clinically associated.Mouse experimental periodontitis modelas and preliminary clinical studies show that diabetes drug metformin has beneficial effects on the periodontium, the tooth associated tissues including bone. This study has analyzed the effect of metformin on clinically relevant cell models: periodontal ligament derived fibroblasts (PDLF) that were either steered into osteogenesis pathways or into osteoclastogenesis pathways, together with peripheral blood monocnuclear cells (PBMCs) under continual exposure to metformin. The effect of metformin on osteoclast formation was further assessed with CD14 + monocytes that were subjected to osteoclast differentiation with osteoclastogenesis driving cytokines M-CSF and RANKL. Our results all indicate an inhibitory effect on osteoclast formation, which could, in a broader sense, be the mechanism that explains clinically observed bone improvement in metformin users.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Diabetes Medication Metformin Inhibits Osteoclast Formation and Activity in In Vitro Models for Periodontitis

    doi: 10.3389/fcell.2021.777450

    Figure Lengend Snippet: Graphical abstract. Diabetes and periodontitis are clinically associated.Mouse experimental periodontitis modelas and preliminary clinical studies show that diabetes drug metformin has beneficial effects on the periodontium, the tooth associated tissues including bone. This study has analyzed the effect of metformin on clinically relevant cell models: periodontal ligament derived fibroblasts (PDLF) that were either steered into osteogenesis pathways or into osteoclastogenesis pathways, together with peripheral blood monocnuclear cells (PBMCs) under continual exposure to metformin. The effect of metformin on osteoclast formation was further assessed with CD14 + monocytes that were subjected to osteoclast differentiation with osteoclastogenesis driving cytokines M-CSF and RANKL. Our results all indicate an inhibitory effect on osteoclast formation, which could, in a broader sense, be the mechanism that explains clinically observed bone improvement in metformin users.

    Article Snippet: For osteoclastogenesis assays, peripheral blood mononuclear cells (PBMCs) derived from a buffy coat (Sanquin, Amsterdam) were added as source containing osteoclast precursors.

    Techniques: Derivative Assay